Heart rate variability(HRV) was estimated for 160 patients stayed in ICU using their stored ECGs. Investigation of relationship between HRV time series and whether they developed sepsis or not reveals that HRV tends to decrease gradually and then increase afterwards from 12-36 hours before septic shock development. Real-time monitoring of HRV is expected to predict sepsis development.
This is a new technology to visualize tissue characterization with MRI. By using both two images obtained with/without an off-resonance RF pulse and the proposed calculation equation (so-called ECR : equivalent cross-relaxation ratio), we can get an new quantitative image which is very sensitive to the tissue condition.
Recently, a rapid and efficient protocol for inserting an objective gene into a vector with homologous recombination has been developed. Retrovirus vector, which is widely used for gene therapy, has homologous sequences in itself, and homologous recombination cannot be used for inserting an objective gene into it. We have developed a retrovirus vector that we can insert an objective gene to with homologous recombination.
Development of a humanized mouse model of pulmonary fibrosis
三重大学 大学院医学系研究科 生命医科学専攻 教授 ガバザ エステバン
Gabazza Esteban, Mie University
今まで、TGF-β１遺伝子の一部をマウスの肺に導入した報告はあるが、本発明はヒト全長TGF-β１遺伝子の発現により生体内での活性化を含めた自然発症モデルである。マウスsurfactant protein Cのプロモーター領域に配置されているヒトtransforming growth factor(TGF)-β1の全長遺伝子が導入されたトランジェニック(TG)マウスである。本TGマウスでは肺特異的にTGF-β1遺伝子が発現され、生後１０週齢から自然発症的に肺線維症を認める。
Novel "Tumor-hoiming peptides", which are highly absorbable to target cancer tissues/ cells were developed from the unique random peptide library. They show lower incorporation to normal human tissues in contrast to the target tumor tissue. Thus, these are considered to be available to various medical technologies such as cancer diagnostics, cancer therapeutics, as an non-invasive nanobio-tool.
Non-viral nanocarrier bio-nanocapsules harboring virus-derived infection mechanism were modified with anti-dendritic cells (DCs) antibodies, and then conjugated with proteinous or DNA antigens to formulate new vaccines. After administrating the complexes to mice, they accumulated to various DCs rapidly, and thereby elicited immunological responses more effectively than conventional vaccines containing same antigens (Matsuo et al., Int. J. Nanomed. 2012; 7: 3341-50).
We have successfully developed a novel and simple method of large scale production of adipose-derived stem cells. These cells have strong immunosuppressive properties, and can be applied for the therapy of autoimmune diseases including various collagen diseases and nephritis. I want to pursue partnering with medical device and pharmaceutical manufacturers in order to promote standardization of the cell reagent.