Hydroxytyrosol acetate is a polyphenol present in olive fruit and oil at a concentration similar to hydroxytyrosol. The effect of hydroxytyrosol acetate on adipogenesis, lipolysis and glucose consumption in 3T3-L1 cells was investigated. Hydroxytyrosol acetate treatment effectively inhibited 3T3-L1 differentiation and lipid accumulation in a dose dependent manner with less cell toxicity. Inhibition of adipogenesis was associated with downregulation of PPARγ, C/EBPα, SREBP-1c and their downstream target genes (GLUT4, CD36 and FAS) as revealed by qRT-PCR. On another hand, hydroxytyrosol acetate dose-dependently activated glycerol release in fully differentiated 3T3-L1 adipocytes. More investigation of the in vitro activities of this polyphenol showed that hydroxytyrosol acetate has the capability to increase glucose consumption and glucose uptake in 3T3-L1 adipocytes and C2C12 myotubes. These results indicate that Hydroxytyrosol acetate, as well as Hydroxytyrosl itself, possibly be the useful material for the prevention and improvement of lifestyle-related diseases.
We already identified and cloned mannitol synthesizing enzymes from red algae. The introduction and expression of these enzymes into fresh water microalgae and microorganisms could give high salt and stress tolerance in transformants. This technology will enable us to cultivate useful microalgae and microorganisms with seawater instead of freshwater. This technology will be very important in desert, arid land and countries which water shortage problem already appeared.
The project which is to observe various targets, i.e., agricultural field, open-pit of mining, and damaged buildings, by using intelligent UAV (unmanned aerial vehicle) is introduced. Especially, this presentation introduces the automatic panorama images generation from videos taken by UAV, and its application to breeding experimentations. The problem of agriculture is that breeding processes are strongly depended on the subjective evaluation of breeders, and there is great variability among their evaluation. Also it is quite difficult to share the knowledge to perform the effective breeding in the agriculture. In order to solve these problem, the project produces the images of plants taken by UAV and it leads to able to perform the quantitive evaluation instead of subjective evaluation.